Title

Thick Slice Clarity for Localization of Novel Neuroactive Target Gene Products

Authors

Eva C. Ping, Parkland College
Michael C. Saul, University of Illinois at Urbana-Champaign
Lisa Stubbs, University of Illinois at Urbana-ChampaignFollow

Document Type

Poster

Publication Date

Summer 2017

Abstract

This poster describes the Thick Slice CLARITY technique, an alternative to the whole tissue or whole body clarity technique. The CLARITY technique is a tissue clearing method allowing for both structural and molecular analysis of a tissue sample, but the method applied to whole tissue or body is very expensive and time consuming. Thick Slice CLARITY applies the same principles but to a 200 micrometer slice of brain tissue. This method, used in conjunction wit standard fluorescent staining methods and imaging via confocal microscopy, has allowed for the colocalization of GPR3 with neurons in the mouse striatum.

Rights

Copyright is owned by the creators of this work.

Comments

Research featured in this poster has been conducted by at the Carl R. Woes Institute for Genomic Biology at the University of Illinois at Urbana-Champaign.

Recommended Citation

Ping, Eva C.; Saul, Michael C.; and Stubbs, Lisa, "Thick Slice Clarity for Localization of Novel Neuroactive Target Gene Products" (2017). PRECS student projects. 4.
https://spark.parkland.edu/precs_student/4