Publication Date
Summer 2025
Abstract
Rhizobium rhizogenes is a plant pathogen that is used to generate transgenic “hairy roots” in a variety of plant species. However, it was observed that repeated freeze-thaw cycles decreased transformation efficiency. Therefore, the aim of this experiment was to determine if transformation efficiency could be recovered by re-culturing the bacteria. Rhizogenes was thawed either 1 or 5 times and then regrown using either solid or liquid media. We found that freeze-thawing alone was not enough to decrease transformation efficiency, as the 1 thaw and 5 thaw treatments both yielded a high number of transformed plants. Future work will investigate other factors, such as increased time and severity of thaw on transformation efficiency.
Rights
Copyright is owned by the creator of this work.
Recommended Citation
Graner, Jill; Cha, Ellen; Larkin, Justin; and Hind, Sarah, "Impacts of culture conditions for Rhizobium rhizogenes-mediated hairy root transformation of tomato" (2025). Parkland Science Scholars. 12.
https://spark.parkland.edu/science_scholars/12
Comments
Cohort 4