Publication Date
Spring 2024
Abstract
Introduction
- Plant transformation is the process of integrating foreign DNA into the genome of a host organism, so that the DNA will be permanently expressed in the organism.
- The plant pathogen Rhizobium rhizogenes inserts its own DNA into the host genome, leading to the formation of structures called hairy roots.
- Plant transformation using R. rhizogenes is cheaper and less labor intensive than other transformation methods [1]. However, only the roots are transgenic, so these changes are not inherited by the next generation.
- Previously, we modified existing hairy root transformation procedures [1,2] to work for our research system. However, we still needed to optimize several additional parameters.
Rights
Copyright is owned by Parkland College.
Recommended Citation
Rattin, Kevin; Larkin, Justin; and Hind, Sarah R., "Optimizing a Hairy Root Transformation Pipeline in Pumpkin Utilizing an Ultraviolet Fluorescent Reporter" (2024). Parkland Science Scholars. 4.
https://spark.parkland.edu/science_scholars/4
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