Optimizing a Hairy Root Transformation Pipeline in Pumpkin Utilizing an Ultraviolet Fluorescent Reporter

Authors

Kevin Rattin, Parkland College
Justin Larkin, University of Illinois at Urbana-Champaign
Sarah R. Hind, University of Illinois at Urbana-Champaign

Publication Date

Summer 2023

Abstract

Introduction

• Plant transformation is the process of integrating foreign DNA into the genome of a host organism, so that the DNA will be permanently expressed in the organism.
• The plant pathogen Rhizobium rhizogenes inserts its own DNA into the host genome, leading to the formation of structures called hairy roots.
• Plant transformation using R. rhizogenes is cheaper and less labor intensive than other transformation methods [1]. However, only the roots are transgenic, so these changes are not inherited by the next generation.
• Previously, we modified existing hairy root transformation procedures [1,2] to work for our research system. However, we still needed to optimize several additional parameters.

Rights

Copyright is owned by Parkland College.

Comments

Cohort 2

Recommended Citation

Rattin, Kevin; Larkin, Justin; and Hind, Sarah R., "Optimizing a Hairy Root Transformation Pipeline in Pumpkin Utilizing an Ultraviolet Fluorescent Reporter" (2023). Parkland Science Scholars. 4.
https://spark.parkland.edu/science_scholars/4